Surface Plasmon Resonance

Surface plasmon resonance P. Anton van der Merwe 1. 2. 2.1. INTRODUCTION PRINCIPLES AND APPLICATIONS OF SURFACE PLASMON RESONANCE. Principles 3 4 4 5 5 7 2.2. Applications 2.2.1. What SPR is safe(p) for 2.2.2. What SPR is not good for. 3. 3.1. 3.2. 3.3. common PRINCIPLES OF BIACORE EXPERIMENTS A typical try out Preparation of materials and buffers Monitoring the Dips 8 8 9 9 4. 4.1. LIGAND Direct versus indirect immobilization 10 10 11 11 12 12 12 14 15 16 16 17 17 18 19 19 4.2. covalent immobilisation 4.2.1. A general betterment 4.2.2. Choice of chemistry 4.2.3. Prepare the protein 4.2.4. Pre-concentration 4.2.5. Amine coupling 4.2.6. conversion 4.2.7. Adjusting the immobilisation conditions. 4.3. Non-covalent immobilisation (ligand capture) 4.3.1. Using an existing strategy 4.3.2. ontogenesis a impudent strategy 4.4. 4.5. 4.6. Activity of immobilised ligand Control surfaces Re-using demodulator chips 5. 5.1. ANALYTE Purity, ac tivity and concentration 20 20 -1- 5.2. 5.3. 5.4. valence refractive index effect and control analytes Low molecular freight analytes 20 21 22 6. 6.1. 6.2. QUALITITATIVE ANALYSIS; DO THEY act? Positive and negative controls Qualitative comparisons using a multi-valued analyte 22 22 23 7. QUANTITATIVE MEASUREMENTS 23 24 24 24 26 27 27 30 30 31 33 34 35 35 37 7.1. Affinity 7.1.1. Concepts 7.1.2. Experimental chassis 7.1.3. info digest 7.1.4. Controls 7.1.5. Non-linear Scatchard Plots 7.2. Kinetics 7.2.1. Concepts 7.2.2. Experimental design 7.2.3. Data analysis 7.2.4. Controls 7.3. 7.4. 7.5. Stoichiometry Thermodynamics energizing energy 8. 9. 9.1. CONCLUSION APPENDIX Physical basis of SPR 38 38 38 39 39 40 41 9.2. Samples and buffers 9.2.1. Guidelines for preparing samples and buffers for use on the BIAcore 9.2.2. Standard buffers 9.3. Additional information 10. REFERENCES 11. ACKNOWLEDGEMENTS -2- 41 42 12. GL OSSARY 13. FIGURE 1....If you destiny to ge! t a full essay, order it on our website: BestEssayCheap.com

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